Single Cell Genomiphi Dna Amplification Kit
At very low levels of input dna successful amplification relies on reagent quality.
Single cell genomiphi dna amplification kit. Single cell genomiphi dna amplification kit is sensitive up to amplification of 1 fg of gdna. Amplification from a single cell reach threshold at around 10 cycles 1 cycle 5 minutes. Single cell genomiphi dna amplification kit combines the unique capabilities of phi29 dna polymerase with an optimised formulation to amplify genomic dna from 1 to 1000 cells without background interference pcr reagents are kits that include researcher supplied components such as purified nucleic acid templates and oligonucleotide primers along with components available through vwr such as deoxyribonucleotides dntps and dna polymerase. Whole genome amplification of genomic dna from as little as a single cell.
Figure 3 shows the real time amplification of single female human cells with a ntc. Single cell genomiphi dna amplification kit combines the unique capabilities of phi29 dna polymerase with an optimized formulation to amplify genomic dna from 1 1000 cells without background interference.